Q: What is the cost?
A: See our price list for current pricing.
Q: Are there any additional discounts?
A: If you have 10 or more plates in one month, then you qualify for a volume discount. See our price list for current pricing.
Q: Why do I have to submit 15 µl? I thought you only used 6.
A: The larger volume provides the facility with extra sample for performing repeat reactions should they be necessary without the time delay of requesting a new submission. The increase to 15 µl ensures that 2 aliquots of 6 µl each can be accurately obtained.
Q: How do I analyze my data?
A: Compressed instrument output files are posted in .zip format to our secure website for retrieval by registered users of the investigator’s account. After zip files are extracted using winzip or pkzip, PC users can view data using any of several free software packages such as Sequence Scanner (ABI) or Chromas. ABI no longer supports MAC format, so MAC users will need to purchase a program such as 4Peaks (Nucleobytes) or Sequencher (GeneCodes Corp) (also available for PC).
Q: Why do I need to leave a well empty on a full plate?
A: The Facility uses that well for a control reaction. This assures that problems associated with the instrument are detected. If a sequencing problem occurs in the control reaction, your samples will be rerun free of charge, at the Director's discretion.
Q: Why can I only run 2 plates a day?
A: The 2-plate/day limit ensures that all investigators have equal access to the instrument. If there is a day when no other plates are in the queue, then up to 8 plates may be run from a single investigator.
Q: My sequences look awful. Why am I being charged?
A: The Facility includes 2 control samples in each set of sequencing reactions. These control samples are used to ensure that excess terminators were removed, that the resolution was adequate and that the computerized data extraction proceeded normally. If a problem is detected in any of these, customer samples are repeated free of charge. If, however, no problems are detected in the control samples, the customer is billed for their samples. Our experience is that most problems arise from the quality or quantity of the template DNA. Looking at the electropherogram may give hints as to the problem. Please contact GRC for assistance in troubleshooting your sample preparation and primer design.
Q: My sequences look awful. What went wrong?
A: Our experience is that most problems arise from the quality or quantity of the template DNA. Please review the guidelines for sequencing primer design and sample preparation. You may also find it useful to review the guidelines for template preparation provided in ABI’s BigDyev3.1 protocol Chapter 2. Looking at the electropherogram may give hints as to the problem. Please contact GRC for assistance in troubleshooting your sample preparation and primer design.
Q: Can I do my own sequencing reactions and have you run them?
A:Yes, you can do your own sequencing reactions. A capillary run is $150 per plate. The sample submission guidelines provide details on doing your own sequencing.
Q: When will my data be ready?
A: Capillary runs are typically available within 2 business days. Samples for sequencing reaction will be available within 3 business days.