Genomics Research Core

DNA Methylation

The Genomics Research Core offers several technologies to support the analysis of DNA methylation patterns. These include:

lllumina HumanMethylation Arrays

  • single nucleotide resolution for 450K CpG islands
  • covers 99% of RefSeq genes and 96% of CpG islands
  • our service includes bisulfite conversion of your DNA

Next-Generation Sequencing

  • enrichment methods can isolate methylated genomic regions based on CpG methylation density
  • bisulfite whole genome sequencing provides a means to analyze the methylation rates across the genome

Epityper

  • most effective for analysis of a small number of CpG sites on a large number of samples
  • valuable for validating genome-wide studies, monitoring tumor or developmental changes and investigating heritable methylation changes

Limited support is available for this platform. Please contact Genomics Research Core  to discuss your project if you plan to utilize this technology.

SABiosciencs Epitect or Zymo qMethyl

  • qPCR based
  • targets specific CpG islands¬†
  • disease and pathway arrays or custom designs are available

We can assist with custom assay designs. Please contact Genomics Research Core  to discuss your project

Bisulfite Sanger Sequencing

  • qualitative focused analysis of CpG sites.

Please contact Genomics Research Core to discuss your project needs and which of these technologies might be most helpful to you in achieving your research aims.